FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480010828
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG1612700.010.936135140-144
GTCGCCG3439350.010.621948140-144
GGGGGGG49352900.010.478992140-144
GTCGGCG774950.010.450015140-144
CGCCGGA1500850.010.076782140-144
GGTCGCC3723700.010.016192140-144
TCGGTGG7600900.09.946517140-144
GTGGTCG4427200.09.925415140-144
TGGTCGC3568250.09.899742140-144
CGCCGTA1620500.09.848004140-144
TCGCCGT1866650.09.700197140-144
AGGGGGG3833200.09.573574140-144
CGGTGGT5980650.09.466314140-144
CTCGGTG7831050.09.433707140-144
TCTCGGT7853000.09.3402405140-144
CCGGATC1295850.09.106098140-144
GCCGGAT1433600.09.081188140-144
GCCGTAT1712450.09.071483140-144
TCGGCGG552750.08.975275140-144
ATCGCGG450250.08.972043140-144
Produced by FastQC (version 0.11.5)