FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379203543
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA2047650.061.3155062
CGAGTAG1998650.060.7516173
GTAGCTG7480350.059.8896566
AGTAGCT7781150.058.5095755
CGGAGGT2013300.058.2450757
CTCGGGA2056750.055.372855
CCCGAGT2488650.054.8291441
GCTGGGA15056500.054.565879
TCGGGAG2328950.052.4745986
GAGTAGC5438800.052.3929634
TTGGGAG11862950.052.356276
GGAGGCT11749800.051.307799
GCGGAGG2397700.050.6878436
TACTCGG2388150.048.6435173
TAGCTGG9460950.048.6238447
GGCGGAG3084850.048.1345145
GGGAGGC15661400.047.560868
CTACTCG2429050.047.169292
GGAGGTT5908050.044.9948968
AGGCGGA3278450.044.7647974
Produced by FastQC (version 0.11.5)