FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350849045
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT1517750.055.3371547
CGAGTAG1610400.050.169613
CCGAGTA1702050.049.520342
GTAGCTG6053200.048.3037346
CTCGGGA1634050.047.681195
GCGGAGG1803950.047.599356
GGCGGAG2376400.046.73035
AGTAGCT6410650.046.318565
TCGGGAG1827900.045.6091046
GCTGGGA12015100.045.4671679
TTGGGAG9755550.044.6202246
CCCGAGT2198250.044.32631
CTACTCG1789100.043.6313362
TACTCGG1843200.043.6064763
GGAGGCT9401850.043.5424169
AGGCGGA2587700.042.745674
GAGTAGC4404800.042.042274
GGGAGGC12485550.041.345668
GGAGGTT4722950.041.1399278
TAGCTGG7666600.039.358177
Produced by FastQC (version 0.11.5)