FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350849045
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA1651200.054.368722
CGAGTAG1606700.053.5130423
CGGAGGT1651800.053.2997067
GTAGCTG6140800.050.268096
CTCGGGA1688950.048.7856835
AGTAGCT6448200.048.4758075
GCTGGGA12170950.047.937639
CCCGAGT2157850.047.799571
TCGGGAG1905050.046.4529846
CTACTCG1781600.046.450462
TACTCGG1816950.046.4018823
TTGGGAG9965400.046.1524626
GCGGAGG1975450.045.6572536
GGAGGCT9596600.044.9472129
GGCGGAG2526450.044.926995
GAGTAGC4399500.044.5378154
GGGAGGC12774200.042.6949168
GGAGGTT4892050.041.6180958
AGGCGGA2713950.041.5974124
TAGCTGG7752150.041.165787
Produced by FastQC (version 0.11.5)