FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366490711
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA1969000.061.461612
CGAGTAG1919500.060.7821543
CGGAGGT1928750.057.776567
GTAGCTG7333850.057.5931366
AGTAGCT7661850.056.0122575
CCCGAGT2422750.055.6980861
GCTGGGA14613150.053.9596949
GAGTAGC5215000.051.4236454
CTCGGGA1992850.051.1794245
TTGGGAG11447350.050.269896
GCGGAGG2281250.050.1589056
TCGGGAG2243550.048.6120576
GGAGGCT11139000.048.2378929
GGCGGAG2932600.047.7232865
TACTCGG2202850.047.4201473
CTACTCG2187700.046.9609152
TAGCTGG9282700.046.915847
GGGAGGC14995950.045.4969838
GGAGGTT5711300.044.5920228
AGGCGGA3155900.043.8536384
Produced by FastQC (version 0.11.5)