FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744439631
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG399344700.013.819051140-144
CGCCGTA734150.010.16641140-144
TGGGGGG38211850.09.770923140-144
GGGGGGT15056750.09.471968140-144
GTGGGGG46585000.09.054046140-144
GTCGCCG1209300.08.878117140-144
TCGCCGT945050.08.617177140-144
AGGGGGG7798150.08.48265140-144
GGGGGTG23458250.08.386591140-144
GGTGGGG50559700.08.1648035140-144
GGGTGGG47346400.08.066858140-144
GGGGTGG25689150.07.62211140-144
TGGTCGC1442850.07.6137824140-144
GCCGTAT1039450.07.5374684140-144
GGTCGCC1473550.07.4588346140-144
GTGGTCG1825400.07.212886140-144
AAGGGTG44498850.06.975201140-144
GAAGGGT45153100.06.872213140-144
AGGGTGG39373300.06.5491333140-144
GTATGGA55579650.06.5209284140-144
Produced by FastQC (version 0.11.5)