FastQCFastQC Report
Wed 11 May 2022
EGAF00005726138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29873848
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG7338612.456533219289326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCC846460.28334481717922644TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTCT523910.17537412656046183TruSeq Adapter, Index 6 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTC468400.15679265690847727TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTAA436320.14605416751132966TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTCA434190.14534116930634447TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTTA411550.13776263439514053TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTTT392650.131436030604427TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTGC385220.12894890541051154TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTAC374490.12535713511028107TruSeq Adapter, Index 6 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTA364770.12210345316077124TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTAT361560.12102893473917387TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTCC349870.11711581313528809TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTTC346090.1158504923771454TruSeq Adapter, Index 6 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTT342540.11466216203550343TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTATCCTTG308860.10338808713226365TruSeq Adapter, Index 6 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)