FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005726202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29877708
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4697491.5722390753668252Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACAC916030.306593129566699TruSeq Adapter, Index 1 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACACGT532890.17835705469777TruSeq Adapter, Index 1 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACACGC363750.12174628656254356TruSeq Adapter, Index 1 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACACGG363200.12156220283028403TruSeq Adapter, Index 1 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACACGTT357870.11977826411584182TruSeq Adapter, Index 1 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACACGTG338200.1131947604548515TruSeq Adapter, Index 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)