FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29539137
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1473530.4988398950179215TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA1401980.4746177926592777TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT176300.040.33463744
AGATCGG213150.032.783991
CGTATGC30350.026.1640971
TATGCCG32350.024.2085443
ATACGAG24150.023.04448116
GCATACG25450.021.95408614
TCTCGTA335250.020.51092343
CCGATCT335800.020.47802239
CCCGATC336900.020.41768838
CATACGA27600.020.40329615
GTCCCGA355150.020.28756536
TCCCGAT341300.020.28574237
CGTCCCG355300.020.28519235
GATCGGA363600.020.2560391
ACCCGTC363800.020.1797232
CGGCATA28250.020.01200912
ATCTCGT345300.019.82466142
ACACGTC369400.019.81216613
CACCCGT373200.019.6539931
ACGTCTG375300.019.45937715
Produced by FastQC (version 0.11.5)