FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51160382
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG60400.022.4335940
TTGAACG76100.019.1344157
ACGGTAA70850.019.06256744
AACGGTA72300.018.71055643
CGAACGG76950.018.03703541
TCGTATG45950.017.80780244
GTCGAAC80700.017.36333739
CTCGTAT48600.016.65564343
GCCTAAC82950.016.6277124
GAACGGT83200.016.60290542
AGTCGAA85150.016.37902538
GAACGCT90150.015.7619189
AACGCTG89300.015.69026510
ATTGAAC116150.014.411556
TATCTCG56100.014.38991440
GATCGGA58000.014.1871162
GGCCTAA104600.014.02713923
CTCAGAT220050.013.76741
TCGACTA29650.013.72460744
GAACGTA28450.013.6862814
Produced by FastQC (version 0.11.5)