FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45134945
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41050.020.68397944
TCGAACG50950.020.55035440
TCGTATG43900.019.44135943
CTCGTAT46300.018.48088542
TTGAACG64000.017.8717047
ACGGTAA59950.017.5387544
AACGGTA63400.016.5496443
AGTCGAA66650.015.97448138
GTCGAAC67350.015.77538739
CGAACGG66800.015.77276741
AACGCTG72400.015.61592210
GAACGGT68900.015.51569342
GATCGGA56350.015.5005211
GCCTAAC69500.015.06611524
GAACGCT77350.014.47449
TCGACTA26600.013.97542344
ACACGTC65550.013.72469913
ATTGAAC101250.013.6647456
GGCCTAA87850.013.145946523
CTCAGAT189250.012.9346551
Produced by FastQC (version 0.11.5)