FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35545922
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT6044961.7006057685041904TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA3435080.9663780841020243TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG427200.038.9744261
CTCGTAT801500.036.4948844
GATCGGA1099250.027.2349431
ATCGGAA1129800.026.4495892
TCGGAAG1127850.026.4268073
ACACGTC1179200.025.29374313
GCACACG1190300.025.12421811
CGGAAGA1195450.025.0481784
ACGTCTG1189550.025.0133315
CACGTCT1192850.024.98401614
CACACGT1198700.024.93545312
TCTCGTA1169400.024.52247443
AATCTCG1170900.024.43948641
ATCTCGT1177200.024.3436542
AGAGCAC1237250.024.2739738
GAGCACA1238050.024.2529249
ATACGAG90200.024.1928116
AGTCAAC1226400.024.09029634
AGCACAC1244050.024.08114210
CGTCTGA1240650.024.007716
Produced by FastQC (version 0.11.5)