FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37145169
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA2213560.5959213700171886TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1156230.3112733179380608TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT143300.037.21142244
AGATCGG288350.036.6345521
CGTATCT371700.027.15467840
TATCTCG377350.026.86346442
GTTCCGT392250.026.54874636
TTCCGTA393900.026.44867137
GATCGGA402950.026.2383042
CCGTATC388950.025.978439
TCTCGTA392300.025.91239244
AGTTCCG404600.025.80368835
ACACGTC412050.025.67643414
ATCTCGT399900.025.48047643
ACGTCTG419050.025.24458516
CACGTCT423800.024.94108215
GTATCTC410500.024.70548241
TCGGAAG432250.024.48224
CACACGT434900.024.33748813
ATCGGAA439000.024.1007863
TCCGTAT423750.023.96255138
GCACACG444350.023.88425412
Produced by FastQC (version 0.11.5)