FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29559017
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC343100.1160728721120868TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC51850.031.52305444
TCGAACG48900.025.01397940
TCGACTA34100.024.2561444
TTGAACG54400.023.9766337
TCGTATG71650.022.71987743
ACGGTAA56250.021.93959244
AACGGTA56400.021.88135343
AACGCTG58800.021.58387410
CTCGTAT75500.021.50318342
GTCGAAC58050.021.29837839
GAACGCT61650.020.8715029
CGAACGG61000.020.12394741
AGTCGAA62000.020.08159838
GAACGGT62050.019.99542642
GCCTAAC64200.019.63631424
ATCGACT42700.019.42244543
TGAACGC70000.018.6961128
ACACGTC90350.018.62368613
AATCTCG88500.018.27075839
GATCGGA93000.018.0040051
Produced by FastQC (version 0.11.5)