FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005726227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35370900
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA10060262.8442194006937904TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT7629292.1569397442530445TruSeq Adapter, Index 15 (97% over 40bp)
CAAGCAGAAGACGGCATACGAGATTCTGACATGTGACTGGAGTTCAGACG740230.20927655219403518RNA PCR Primer, Index 40 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)