FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41525324
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2230420.5371228409921618TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1202620.28961122615202234TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC145800.038.4436144
AGATCGG283950.037.753991
TCGTATG383950.027.59269144
CTCGTAT387350.027.1063943
GATCGGA402800.026.6981262
ACACGTC407450.026.26867914
AATCTCG396300.025.84074840
ACGTCTG414200.025.83233516
TCTCGTA399450.025.67979242
CACGTCT418800.025.54346815
ATCTCGT403450.025.44234741
ATCGGAA427100.025.1694723
CACACGT428400.025.03543913
TCGGAAG434450.024.763824
GTAATCT418650.024.51184538
GCACACG445300.024.23842212
TAATCTC437750.023.43900939
CGTCTGA460100.023.29400317
CTTGTAA456400.023.25064735
TGTAATC474250.021.73092737
Produced by FastQC (version 0.11.5)