FastQCFastQC Report
Sun 5 May 2024
EGAF00005726230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35762962
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2166940.6059173733987694TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1700510.47549473111315554TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)