FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35762962
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2166940.6059173733987694TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1700510.47549473111315554TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG235150.035.4779551
CGTATGC326950.032.4906344
GATCGGA473100.022.9447061
ATACGAG26600.021.9978316
ATCGGAA495200.021.8181882
TCGGAAG503100.021.3891013
TCGTATG502000.021.16983243
ACACGTC508550.021.08940513
CTCGTAT501800.020.98576442
GCATACG29350.020.8339214
GTCACGA510300.020.81475629
ACGTCTG517200.020.71331815
AGTCACG514500.020.6532528
TCGACTA38600.020.63020344
CACGTCT519850.020.6140614
ACGATCA517850.020.50882132
TCACGAT520100.020.4225830
CACACGT525700.020.40140312
CACGATC521100.020.3400931
GCACACG533250.020.21151711
Produced by FastQC (version 0.11.5)