FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46348895
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51400.025.84833144
TCGAACG60950.021.6897640
TTGAACG73650.019.6216017
ACGGTAA70950.018.81893244
AACGGTA74000.018.01342643
CGAACGG77450.017.38108841
GCCTAAC79450.017.05538224
AACGCTG82700.016.8626410
AGTCGAA80100.016.80727638
GTCGAAC79600.016.8018739
TATCTCG78400.016.66546241
GATCGGA82850.016.3623981
GAACGCT86950.016.0890129
GAACGGT84000.015.92118942
GTCACGA85150.015.88740329
TCGACTA25050.015.63043144
AGTCACG88750.015.26782428
ATTGAAC111400.015.2630496
ACACGTC91600.014.84025613
CACGAGT92550.014.61771231
Produced by FastQC (version 0.11.5)