FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38226013
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1087760.2845601501783615TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG505950.13235751267075643TruSeq Adapter, Index 5 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC160900.031.97828744
AGATCGG101700.025.5494691
GATCGGA212000.025.0748731
ACACGTC218750.023.99137713
TCGTATG216500.023.76588243
TCGACTA46400.023.70462444
CTCGTAT218700.023.35615242
TCGAACG59550.023.1259340
ACGTCTG227000.023.07364815
CACGTCT231350.022.76079414
TCGGAAG243250.021.7752193
ATCGGAA242950.021.6700462
CACACGT244150.021.5585112
ATCTCGT235500.021.05569640
TTGAACG70950.021.0500167
TCTCGTA235600.021.03714841
ACGGTAA66600.020.70972644
GCACACG257550.020.58195111
AACGGTA68350.020.21169543
GTCGAAC71750.019.31625739
Produced by FastQC (version 0.11.5)