FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39664564
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3332260.840110079112429TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2440120.6151889127030364TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC304450.039.34258344
AGATCGG410000.039.2912371
TCGTATG664350.023.92334444
GATCGGA676950.023.8292482
CTCGTAT665900.023.75876243
TATCTCG656300.023.48403740
ACACGTC684650.023.41056314
CACTAGC688450.023.13970432
ACGTCTG691700.023.12332316
ACTAGCT690850.023.0134933
CACGTCT699300.022.90664715
ATCTCGT675600.022.8877141
TAGCTTA697750.022.7900735
TCACTAG702550.022.72693831
TCGGAAG712350.022.6801514
CACACGT709750.022.62607413
TCTCGTA684800.022.59253542
ATCGGAA715450.022.5788353
GTCACTA709000.022.57777230
CTAGCTT711950.022.29478834
Produced by FastQC (version 0.11.5)