FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40901599
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC490210.11985106010158673TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72550.031.98878944
TCGTATG99350.023.35990543
CTCGTAT104600.022.08253342
GATCGGA113400.020.9297181
TATCTCG107050.020.81736439
AGTCACG114800.020.44780528
TCGACTA38850.020.32760244
TCGAACG47200.020.22809240
ACACGTC117700.020.03343613
GTCACGC120650.019.45641529
CGCCAAT123450.019.27944433
AGATCGG71300.018.8714141
TCTCGTA120250.018.78828841
ACGTCTG127050.018.63041315
AACGGTA53850.017.81095743
ATCTCGT126850.017.79361240
CACGTCT133400.017.70865214
ACGGTAA55150.017.59040544
TTGAACG62150.017.235757
CACACGT139250.017.18584312
Produced by FastQC (version 0.11.5)