FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45315096
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT58350.027.74562844
TCGAACG63550.022.39460840
CGTACGT98900.021.9543335
TACGTAA100200.021.73540737
AGATCGG99800.021.6053471
ACGTAAT100750.021.11386938
CGTAATC101900.020.68067739
GTACGTA106600.020.40978436
TTGAACG74750.020.3041347
ACGGTAA71350.019.97738644
CCGTACG114300.019.07359334
ACCGTAC115050.018.96820633
AACGGTA75850.018.58915343
CGAACGG77550.018.40810241
GTCGAAC81500.018.11083239
GCCTAAC80700.018.0722824
AGTCGAA80300.017.99849738
GATCGGA122100.017.7127532
AACGCTG85750.017.44300810
TCGACTA28750.017.06179244
Produced by FastQC (version 0.11.5)