FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39214505
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC4312581.0997410269490842TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG405080.10329851160941596TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC496850.041.10017844
GATCGGA547300.038.347781
TCGTATG544500.037.5278743
ACACGTC555050.037.3849513
CTCGTAT544950.037.31181342
AGTCACG555350.036.87011728
ACGGCTA555600.036.81715432
ACGTCTG563250.036.70054615
CACGTCT565600.036.62150214
GTCACGG563700.036.28489729
TCGGAAG584200.035.9431953
TCTCGTA554350.035.88208841
CACACGT580700.035.83968412
TCACGGC573000.035.73821330
CGGCTAC572950.035.68490233
ATCTCGT557150.035.68288440
ATCGGAA589200.035.658762
GCACACG595250.035.18876611
CACGGCT595700.034.3669531
CATCTCG579600.034.2968339
Produced by FastQC (version 0.11.5)