FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005726238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005726238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35994664
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG37350.021.69711740
CTCGTAT28550.020.01805544
ACGGTAA44500.018.32603844
AACGGTA46000.017.63260843
TTGAACG49550.017.3888137
CGAACGG49000.016.89874641
GTCGAAC50600.016.36471739
GCCTAAC50650.016.01657924
GAACGGT53150.015.78684642
AGTCGAA52800.015.68278938
AACGCTG57250.014.3589810
ATTGAAC77550.013.4062556
GAACGCT61650.013.405429
CGTATGC20200.012.8980631
AAGTCGA66350.012.68013837
CTCAGAT152750.012.6190571
GGCCTAA69050.012.57625823
AGTCACG50000.012.35586328
CAAGTCG69800.012.24321436
GAACGTA17600.012.2403734
Produced by FastQC (version 0.11.5)