FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10165459
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC9053638.906267783874785TruSeq Adapter, Index 10 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC180140.17720793522456782TruSeq Adapter, Index 10 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC152060.1495849818488275TruSeq Adapter, Index 10 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATTCGTATGCCG138580.13632439027101484TruSeq Adapter, Index 10 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1218250.041.88371316
TATCGGA22350.041.8754121
TATCTCG1109800.041.87298239
ACACGTC1218600.041.8620413
ACGTCTG1217750.041.83562515
CACGTCT1219800.041.78929514
ATCTCGT1133000.041.7244840
GATCGGA1192050.041.6800541
TCTCGTA1134850.041.66637441
GCACACG1224000.041.6663211
CACACGT1224900.041.6246912
GCTTATC1107500.041.6127836
CTCGTAT1137450.041.5889342
TCGGAAG1215900.041.5421683
CGGAAGA1214200.041.5243534
GTCACTA1160800.041.4927429
ACTAGCT1136700.041.48798432
CACTAGC1137950.041.4816931
ATCGGAA1222250.041.407332
CAGTCAC1213400.041.36959527
Produced by FastQC (version 0.11.5)