FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7747029
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC146191518.87065351117183TruSeq Adapter, Index 9 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC283810.3663468924667766TruSeq Adapter, Index 9 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC240230.3100930692269256TruSeq Adapter, Index 9 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGTCTCGTATGCCG199350.25732445302579865TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTCGTATGCCG157880.20379425454583946TruSeq Adapter, Index 9 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGTATGCCGT119200.15386543667256183TruSeq Adapter, Index 9 (97% over 42bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC110310.14239006979320717TruSeq Adapter, Index 9 (98% over 51bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG85750.11068759391503503TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATGCC81680.10543396700851385TruSeq Adapter, Index 9 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1802600.044.3366171
CGGAAGA1831850.044.2392654
TCGGAAG1834350.044.232943
ATCGGAA1836400.044.1933632
TATCGGA28800.043.848171
GCACACG1877400.043.7093511
AGAGCAC1891550.043.6963928
CACACGT1878750.043.68872512
GAAGAGC1896450.043.6778346
GAGCACA1889900.043.628599
GGAAGAG1904050.043.6216625
ACACGTC1886750.043.6024513
AGCACAC1886900.043.5584510
CACGTCT1888850.043.55698814
CGTCTGA1891850.043.54262516
ACGTCTG1890750.043.51798215
AAGAGCA1905150.043.480127
GTCACGA1820850.043.4105429
CGATCAG1775850.043.40904233
AGTCACG1867950.043.40533428
Produced by FastQC (version 0.11.5)