FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9043988
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC877840.9706337513937435TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG115450.040.00775539
CGTATGC125100.038.21648844
CTCGTAT126800.037.63315242
CGTCTGA143250.037.63103516
TCTCGTA127350.037.39995641
ACACGTC145350.037.118313
ATCTCGT128350.037.07350540
TCGTATG129950.036.94598843
ACGTCTG146050.036.86297215
CACGTCT146250.036.81255714
GTATGCC129900.036.7177345
GTCACAC139200.036.4790829
GCACACG150850.035.7798811
ACACTTG133700.035.27243432
CACACGT153400.035.18510412
CCAGTCA152200.034.9906526
GAATCTC132150.034.9880938
CAGTCAC151800.034.96427527
TCGGAAG153650.034.907833
GATCGGA150350.034.773871
Produced by FastQC (version 0.11.5)