FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11844867
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC218724518.465762426880776TruSeq Adapter, Index 6 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC433360.36586312028661866TruSeq Adapter, Index 6 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC411520.3474247536928865TruSeq Adapter, Index 6 (98% over 51bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC180190.1521249668738366TruSeq Adapter, Index 6 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATTCGTATGCCG164140.13857479362157465TruSeq Adapter, Index 6 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG153080.12923741566705646TruSeq Adapter, Index 6 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGTATGCCGT149250.12600394753271607TruSeq Adapter, Index 6 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2711100.044.0259441
TATCGGA51850.043.6752781
TCGGAAG2774550.043.6247443
ATCGGAA2786250.043.5836682
CGGAAGA2767700.043.5668834
GAAGAGC2847450.043.158936
GGAAGAG2858300.043.15415
AGAGCAC2838950.043.039318
GAGCACA2831800.042.969229
AAGAGCA2863150.042.832697
GCACACG2827000.042.7222511
AGCACAC2841600.042.6674310
CACACGT2835000.042.54137812
ACACGTC2832250.042.50166313
CACGTCT2840650.042.31736814
ACGTCTG2840550.042.27054615
CGTCTGA2845300.042.2517316
TCTGAAC2870250.041.89734618
GTCTGAA2873550.041.8872517
CTGAACT2876900.041.74184819
Produced by FastQC (version 0.11.5)