FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21722735
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTATGC1139690.5246530881125236TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTATG558500.25710390519425846TruSeq Adapter, Index 1 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC142250.041.59414745
CTCGTAT200300.029.20250543
TCGTATG204000.028.8712944
TCTCGTA186350.028.61189742
ATCTCGT191100.027.93603541
CATCTCG197900.027.03141440
CGTCTGA228800.025.87864915
ACACGTC232600.025.50334412
GCACCTC226400.025.13835334
ACGTCTG236450.025.0794414
CACGTCT239900.024.70939313
CACACGT250850.023.67481411
GCACACG254600.023.34377710
CAGTCAC255550.023.24953326
CCAGTCA256350.023.14187425
TCTGAAC264050.022.71525817
AGCACCT253450.022.52651633
GTCACAG266100.022.30250528
ACAGCAC259700.022.28756131
ACTCGTA12800.021.79412342
Produced by FastQC (version 0.11.5)