FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13750492
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC13297749.670737599789158TruSeq Adapter, Index 3 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC276640.2011855284887261TruSeq Adapter, Index 3 (98% over 51bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC230900.16792126419912828TruSeq Adapter, Index 3 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCTCTCGTATGCCG140530.10219997946255305TruSeq Adapter, Index 3 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1590050.044.5054131
ATCGGAA1634650.044.033412
TCGGAAG1635500.044.0256583
CGGAAGA1637500.043.9457784
ACACGTC1669400.043.67611313
CGTCTGA1682150.043.63207616
ACGTCTG1673250.043.5971315
GCACACG1669950.043.59301411
CACGTCT1672850.043.58199714
CACACGT1672250.043.55458512
CATCTCG1585250.043.51375239
AGAGCAC1704500.043.2188878
CTTAGGC1593050.043.1944633
GAAGAGC1709500.043.1898736
ATCTCGT1616400.043.17085640
GAGCACA1697700.043.1349039
AGCACAC1693750.043.10399210
AGGCATC1564800.042.99220736
GCATCTC1584650.042.98043438
ACTTAGG1611200.042.97879432
Produced by FastQC (version 0.11.5)