FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005727587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8181140
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC174625521.34488592054408TruSeq Adapter, Index 1 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC364840.44595252006444086TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGTCTCGTATGCCG305390.37328538565529984TruSeq Adapter, Index 1 (97% over 39bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC281780.34442632689331804TruSeq Adapter, Index 1 (98% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT176740.21603346232921083TruSeq Adapter, Index 1 (100% over 51bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC130290.15925653393047912TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATGATCTCGTATGCC123700.15120142180674087TruSeq Adapter, Index 1 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG120930.14781558560298444TruSeq Adapter, Index 1 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACGATCTCGTATGCC120420.14719220059796068TruSeq Adapter, Index 1 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATTTCGTATGCC120180.14689884294853775TruSeq Adapter, Index 1 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)