FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14446227
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC714450.4945581984832441TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA96950.039.880761
ATCGGAA99100.039.1285482
TCTCGTA91750.038.98996741
CTCGTAT91400.038.9423542
CGTCTGA98600.038.63139316
TCGGAAG100850.038.554343
CGTATGC93050.038.42119644
ACACGTC99050.038.41032413
ATCTCGT94250.037.74077640
TCGTATG95300.037.60864643
ACGTCTG101750.037.54599815
CGGAAGA103700.037.3862534
CACGTCT103550.036.8497514
AATCTCG98150.036.19529739
GCACACG106150.036.11673411
CACACGT106550.035.83333612
GTATGCC102300.035.0351145
GTCACAC114450.032.4190129
AGAGCAC126400.030.6510188
AGCACAC127900.030.1684110
Produced by FastQC (version 0.11.5)