FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005727592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13794416
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG11196528.116704614388896TruSeq Adapter, Index 25 (97% over 44bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG224670.16287025126688945TruSeq Adapter, Index 25 (97% over 41bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG184560.133793268232595TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCGTATGCC152940.11087094952044363TruSeq Adapter, Index 25 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)