FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6268562
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG3452875.508232988682253TruSeq Adapter, Index 19 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG67540.10774400891304894TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA442650.043.7838361
ATCGGAA455000.043.2955442
TCGGAAG454750.043.2352453
CGGAAGA456500.042.975864
ACACGTC466950.042.1637913
GCACACG467800.042.13046311
CGTCTGA467900.042.02562316
CACGTCT469000.041.95037514
ACGTCTG469050.041.93151515
CACACGT470050.041.9096512
GAAGAGC479450.041.7633746
AGAGCAC477850.041.7010888
GAGCACA478350.041.544629
AGCACAC477000.041.5159810
AGTCACG461150.041.50367728
GTCACGT457900.041.4294229
CGTGAAA441350.041.18712633
TCGTATG437000.041.1867445
CACGTGA452200.041.16554331
TCACGTG455850.041.15673430
Produced by FastQC (version 0.11.5)