FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13551813
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2682201.979218573928079TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA336050.043.5389861
ATCGGAA340450.043.121562
TCGGAAG340850.042.8976483
CGGAAGA343400.042.474264
ACACGTC343150.041.6998113
CGTCTGA343350.041.6101516
ACGTCTG346600.041.22647515
CACGTCT346900.041.22309514
CGATCAG337350.041.14579833
GTCACGA340250.041.07224329
AGTCACG341550.040.92295528
ACGATCA341750.040.84005732
CACACGT352450.040.7080112
GCACACG353100.040.70316711
TCACGAT344000.040.66909830
TCTCGTA326900.040.66224741
CTCGTAT327050.040.60919642
ATCTCGT328000.040.50485240
GTATGCC331450.040.1244245
CACGATC349600.039.8779731
Produced by FastQC (version 0.11.5)