FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647716
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG129460.1947435780950931TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT17500.038.43365544
TCTCGTA16900.037.00297543
CGTGATA18700.036.4495936
TATCTCG17300.036.0176641
ATCTCGT18250.034.38902742
ACGTGAT20200.034.18839335
TCGTATG20200.033.8042245
AACGTGA20450.033.44043434
CGTCTGA20750.033.17028816
ACACGTC20950.033.0683513
ACAACGT20700.033.0370632
CACAACG21350.032.0312531
ATCGGAA23600.030.0560152
TCGGAAG23700.029.9287473
ACGTCTG23400.029.4138215
GATCGGA23850.029.2824651
CAACGTG23550.028.84789833
CACGTCT24150.028.68662614
CGGAAGA25350.027.9508364
CACACGT26350.026.54762512
Produced by FastQC (version 0.11.5)