FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14572048
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3742122.5680124029237343TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA491100.043.07961
ATCGGAA504750.042.6119272
TCGGAAG504650.042.5401233
CGGAAGA508250.042.128144
ACACGTC507000.041.85968413
CGTCTGA507500.041.7430816
ACGTCTG508800.041.6050415
GCACACG512800.041.41694311
CACGTCT511900.041.38824514
CACACGT516300.041.0795312
TCGTATG480100.040.85415345
CTCGTAT484200.040.47555544
TCTCGTA484900.040.44496543
AATCTCG475400.040.3871441
ATCTCGT485150.040.34528442
AGAGCAC540250.040.332858
GAGCACA539700.040.2405559
GAAGAGC543550.040.236586
AGCACAC543300.039.8331210
TCTGAAC546150.039.40307218
Produced by FastQC (version 0.11.5)