FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15241817
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG7392734.850294423558556TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA966900.043.1303331
TCGGAAG988800.042.7916343
ATCGGAA991700.042.725482
CGGAAGA990400.042.6270944
CGTCTGA1007750.042.35156216
ACACGTC1006350.042.35011713
ACGTCTG1009650.042.2065515
CACGTCT1011050.042.16145714
GCACACG1011950.042.15577711
CACACGT1015500.041.9840412
TATCTCG914400.041.73386841
AGAGCAC1032800.041.699038
GAGCACA1033700.041.5408449
GAAGAGC1041350.041.5245066
AGCACAC1035950.041.33117310
ATCTCGT933250.041.21395542
TCTGAAC1039550.041.1758718
TCTCGTA934800.041.15043343
CTCGTAT934950.041.11495644
CCAGTCA1022300.041.0721626
Produced by FastQC (version 0.11.5)