FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13025397
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC4600.023.5481361
GGCGACC6150.022.6898882
GCGACCA6750.021.339883
CTCGTAT25750.020.18360744
TCTCGTA25000.019.5291743
CGACCAC7750.018.873284
TCGTATG29700.017.72651945
ATCTCGT28400.017.34961342
CGACGCT7950.016.97969235
ACGACGC8550.015.52499934
GCTAATC32400.015.06793538
CACGACG8850.014.99872833
CGTCTGA37150.014.89756716
ACCGAGA10000.014.6235359
CACCGAG9700.014.6119388
TCCGATC9850.013.93341744
CCACCGA10650.013.731027
ACACGTC40900.013.69667213
GACGCTC10150.013.52102136
TACACGA10850.013.06340831
Produced by FastQC (version 0.11.5)