FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7004161
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15100.015.79068842
TCCGATC2800.015.2628898
TCTCGTA16250.014.95004641
TCGTATG16600.014.77034143
CGTATGC17050.014.38050844
TTCCGAT2853.6379788E-1214.2058997
TCACGAT15600.014.00522635
ATCTCGT17700.013.59823940
GTATGCC19050.012.98789745
CCGATCT3753.8198777E-1111.3962899
CATCACG18450.011.23141533
CACGATC22200.010.34939736
ACGCTCT4253.6925485E-1010.0652491
ACGATCT22250.010.02242937
TTAACGG1850.00369014689.7359461
CTTCCGA4407.057679E-109.7128156
CGTCTGA31150.08.80939316
ACACGTC33100.08.63018213
ACGTCTG33400.08.55266615
GCCGTCT8200.08.5033896
Produced by FastQC (version 0.11.5)