FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11547631
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1861751.6122354446552718TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA234150.041.9831851
ATCGGAA236500.041.5242352
ACACGTC231700.041.5202713
TCGGAAG236750.041.3663523
CGTCTGA231250.041.32866316
CGGAAGA238450.041.0148244
TCACGAT223200.040.96489335
ACGTCTG233700.040.93389515
TCTCGTA221900.040.86994641
CTCGTAT222850.040.5947642
ATCTCGT223800.040.57323540
CACGTCT236900.040.47593714
CATCACG228950.040.3684533
CGTATGC225800.040.1441144
CACACGT239700.040.1345312
GTATGCC226600.040.02224745
GCACACG241400.039.91713311
TCGTATG227400.039.8913443
ACGATCT228550.039.52361337
CACGATC229750.039.31717736
Produced by FastQC (version 0.11.5)