FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10299244
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2227152.1624402723151332TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA282900.042.831891
ATCGGAA293400.042.144192
TCGGAAG294100.041.9597323
CGGAAGA295300.041.6901784
ACACGTC300600.041.1646913
ACGGCTA275550.041.0216432
CGTCTGA301250.040.98744216
CGGCTAC273150.040.97022233
GTCACGG281850.040.9341129
GCACACG303500.040.8158311
CATCTCG278800.040.719839
ACGTCTG303100.040.69897515
CACGGCT279400.040.66456231
TCACGGC280400.040.65613630
CACACGT304500.040.62267712
AGTCACG292800.040.5641428
CACGTCT306450.040.37153214
GTATGCC290450.039.89985345
CGTATGC291300.039.8838344
TCTCGTA290750.039.80451641
Produced by FastQC (version 0.11.5)