FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11621148
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC236910.2038610987485918TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT32150.036.17946242
TCTCGTA33050.035.2623241
GATCGGA37300.034.9637371
CGTATGC33900.034.909144
ATCGGAA38600.033.922532
ACACGTC37850.033.88141613
ATCTCGT34650.033.7639140
TCGGAAG39250.033.418083
CGTCTGA38900.032.90904216
TCGTATG36050.032.76473243
CGGAAGA41450.031.8614944
ACGTCTG41350.030.95917115
CACGTCT42850.029.8754214
GTATGCC40350.029.32883345
AATCTCG42250.028.16965339
GCACACG46050.027.94595711
CACACGT46500.027.53036112
ACTGACC45450.026.68132832
ACCAATC49600.024.2221236
AGAGCAC57350.023.0280558
Produced by FastQC (version 0.11.5)