FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9585046
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC164230.1713398141229578TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG32450.025.85498639
CTCGTAT34450.025.39864542
CGATGTA33200.025.23884434
TCTCGTA34900.025.00670641
CCGATGT33950.024.88032733
ACCGATG34200.024.69845432
ATCTCGT37100.023.76634240
CACCGAT35850.023.5623231
CGTATGC37200.023.52105744
TCGTATG37950.023.11548443
GTCACCG38050.022.10959229
TCACCGA39000.021.62847330
GTATGCC42050.020.91438545
CGTCTGA52050.017.54453716
GTATCTC48000.017.5259138
GATCGGA53550.017.1554431
ATCGGAA56900.016.4619032
TCGGAAG57200.016.3289533
CGGAAGA57100.016.3181344
AGTCACC52850.016.2161728
Produced by FastQC (version 0.11.5)