FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304230
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1023511.1000480426644654TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA129750.041.4717061
CGCCAAT124650.041.4051533
ACGCCAA125800.041.0087632
CTCGTAT123100.040.99439242
GTCACGC126950.040.9560629
TCTCGTA124250.040.75982741
TATCTCG124300.040.74342739
AGTCACG127950.040.67178328
CGTCTGA130450.040.59880416
ATCGGAA133250.040.4379542
ATCTCGT125500.040.3717840
ACACGTC131500.040.36017613
CGTATGC126450.040.10405344
TCGGAAG134400.040.0919463
ACGTCTG133200.039.72683715
TCGTATG128150.039.64227343
CGGAAGA136950.039.4440044
CACGTCT134500.039.3930414
GTATGCC130600.038.8469245
GCACACG138450.038.39915511