FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12791931
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC259380.20276844832887234TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA36100.036.14685441
CTCGTAT36050.036.1345842
CGTATGC36600.035.6530444
GATCGGA39600.035.3308071
ATCGGAA41550.034.0586622
ATCTCGT38650.033.76200540
CGTCTGA40650.033.76115416
TCGTATG38900.033.71853343
CATCTCG39100.033.20081739
ACACGTC41950.033.0903413
TCGGAAG43200.032.7057343
CGGAAGA45500.031.0524774
ACGTCTG44650.030.78702415
CACGTCT46700.029.82097214
GTATGCC45300.029.00454545
GCACACG49650.028.0491311
TCCGATC11500.027.3891288
CACACGT51150.027.2705612
CCGATCT12450.025.1184869
CCAGATC55600.023.91446533
Produced by FastQC (version 0.11.5)