FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5541732
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC535020.9654382420514019TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG71750.035.6431239
TCTCGTA73000.035.3717241
CTCGTAT72950.035.334342
ATCTCGT73900.034.8800740
CGTATGC74500.034.75042744
TCGTATG75050.034.5257343
GTATGCC76100.034.04781745
CTTAGGC77400.033.4973733
GATCGGA82400.033.1770941
TCGGAAG86150.032.1108133
CGGAAGA85900.032.0732464
ACTTAGG81900.031.98690232
GCATCTC80150.031.96372238
AGGCATC80050.031.91124736
CGTCTGA83900.031.8207416
TTAGGCA81350.031.81550834
ATCGGAA86550.031.7935642
GGCATCT81500.031.3987837
CACTTAG84200.031.22044231
ACGTCTG86050.030.99926415
Produced by FastQC (version 0.11.5)