FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14640928
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC412590.28180590738510564TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57600.035.97505642
TCTCGTA55350.035.6076641
ACGGCTA59050.035.4711132
CGGCTAC59200.035.38123733
CGTATGC59300.035.3231444
TCGTATG60050.034.80703743
GTCACGG61250.034.1599729
CGGCGAC8800.034.1057131
AGTCACG61900.033.8012628
ATCTCGT58600.033.59317440
CGACGCT9000.032.9971535
CATCTCG60500.032.83567439
TCACGGC63800.032.75937730
CACGGCT64400.032.45438831
GCGACCA10650.031.487453
CGTCTGA68850.031.33684316
GTATGCC67350.031.1679645
ACGACGC9700.030.61591734
CACGACG9950.030.29889533
GGCGACC11150.030.2773042
Produced by FastQC (version 0.11.5)