FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11686471
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG701050.5998816922576542TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG91650.039.1056645
CTCGTAT91700.038.98620244
CCGTATC87750.038.89409638
TCTCGTA87550.038.80405443
TCCGTAT88550.038.6699137
AGTTCCG93050.038.58900534
TATCTCG89000.038.19664441
CGTATCT89300.038.0928739
CGTCTGA98200.037.80208616
ACACGTC99950.037.34280413
TTCCGTA96300.037.333436
ATCTCGT91400.037.26799442
ACGTCTG101450.036.7019615
GATCGGA104550.036.0664981
CACGTCT104750.035.67458314
TCGGAAG108200.034.881123
ATCGGAA109800.034.4138222
GTTCCGT104900.034.37993635
CACACGT110150.033.92567412
GCACACG111250.033.6104511
Produced by FastQC (version 0.11.5)